Title: Effects of prescriptions of benefiting "qi",removing blood stasis,reducing phlegm and nourishing "yin" on TGF-β1 expression of rats with hepatic fibrosis
Abstract: AIM To observe the effects of prescriptions of benefiting qi,removing blood stasis,reducing phlegm and nourishing yin on TGF-β1 expression of rats with hepatic fibrosis mainly induced by CCl4.METHODS One hundred and thirty male SD rats were randomly divided into normal control group(10 rats),hepatic fibrosis model group,benefiting qi group,removing blood stasis group,reducing phlegm group and nourishing yin group(20 rats per group).Hepatic fibrosis models were made in these experimental groups except for the normal control group giving subcutaneous injection of carbon tetrachloride plus low-protein high-fat diet plus alcohol.Modeling rats were in corresponding groups respectively fed with physiological saline,the single prescription of Astragali Radix plus Atractylodis macrocephalae Rhizoma extract,Chuanxiong Rhizoma plus Curcumae longae Rhizoma extract,Pinelliae Rhizoma plus Sargassum extract,Trionycis Carapax plus Atractylodis macrocephalae Rhizoma extract and the mixture of eight herbs combination.Twelve weeks later,liver tissue samples were collected and analyzed heart perfusion changes of liver tissue structure were observed through the Masson trichrome staining collagen and electron microscope.And RT-PCR and Western Blot methods were used to measure the changes of TGF-β1 gene and protein expressions in each group.RESULTS Compared with the model group,all single prescriptions of benefiting qi,removing blood stasis,reducing phlegm,nourishing yin could reduce the degree of fibrosis of rat liver tissue as well as TGF-β1 gene and protein expression(P0.05).Among them,the benefiting qi method was the worst(P0.05) and nourishing yin method was the best(P0.01),while the two others almost equal(P0.05).Compared with treatments with single prescriptions,the mixture group could much more effectively reduce the degree of fibrosis of rat liver tissue as well as TGF-β1 gene and protein expression(P0.05).CONCLUSION All single prescriptions of benefiting qi,removing blood stasis,reducing phlegm,nourishing yin can reduce the liver tissue fibrosis to different degrees while the mixture group shows more effective.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot