Title: The Separation and Purification of Gentiopicroside from Gentiana
Abstract:Objective To establish a rapid separation and purification method of high-purity Gentiopicroside.Methods Ethanol extract of gentian herbs was roughly divided by AB-8 macroporous resin column with etha...Objective To establish a rapid separation and purification method of high-purity Gentiopicroside.Methods Ethanol extract of gentian herbs was roughly divided by AB-8 macroporous resin column with ethanol-water as gradient eluent. After freeze-drying, 30% ethanol solution eluted fractions was further purified by MCI.The purity of gentiopicroside was detected by HPLC. Results Under this condition, we could get gentiopicroside with a purity more than 97%. Conclusion The method was simple, rapid, low toxicity, and the purity of product was high. This method was suitable for the separation of high purity gentiopicroside and preparation of reference substance gentiopicroside.Read More
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot