Title: Determination of Total Alkaloid and Chelerythrine in Toddalia Asiatica from Different Places in Guizhou
Abstract: [Objective] To determine the contents of total alkaloid and chelerythrine in Toddalia asiatica from different places in Guizhou.[Method] Total alkaloid was determined by back-titrantion method,and chelerythrine was determined by HPLC method,using Diamonsil C18 column(250 mm×4.6 mm,5 μm),and acetonitrile-0.1% phosphoric acid(V/V,28∶72) as mobile phase,with the detection wavelength at 270 nm and flow rate of 1.0 ml/min.[Result] The average recoveries of total alkaloid and chelerythrine were 97.5% and 99.8% with RSD = 2.71% and 0.40% respectively.[Conclusion] This analysis method is accurate,sensitive and reliable to determine the contents of alkaloid in Toddalia asiatica.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot