Title: Content Determination of Sanguinarine and Chelerythrine In Macleaya microcarpa by HPCE
Abstract: Objective:To establish a new method for determination of sanguinarine and chelerythrine in Macleaya microcarpa(Maxim).Method: Sanguinarine and chelerythrine in Macleaya microcarpa were determined by using high performance capillary electrophoresis(HPCE) with berberine as internal standard.The background electrolyte system selected was 25 mmol·L-1 disodium hydrogen phosphate and 25 mmol·L-1 sodium dihydrogen phosphate buffer at pH=7-methanol(2.5∶1);applied voltage,18 kV;temperature,25 ℃.Diode array detector was set at 200 nm.Result: The linear range of sanguinarine and chelerythrine was 0.012 4-0.082 8 g·L-1(r=0.999 8) and 0.018 8-0.125 6 g·L-1(r=0.999 6),The average recovery was 98.7%(RSD 1.01%) and 98.4%(RSD 1.27%).Conclusion: The method is simple,rapid and reproducible,and could be used for content determination of sanguinarine and chelerythrine In Macleaya microcarpa.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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