Title: HPLC-DAD determination of 5 alkaloids in commercial available Kushen's rhizome and root
Abstract: Objective:To determine the respective content of matrine,oxymatrin,sophocarpine,oxysophocarpine and sophoridine in commercial Kushen's rhizomes and roots so as to find out if the total contents of matrine and oxymatrin meet the request of not less than 1% in ChP(Vol.I)2010 Ed.Methods:The procedure was performed on a C18(4.6 mm×150 mm,5 μm) column with a gradient elution of mobile phase A(10 mmol·L-1 ammonium acetate aqueous,adjusted to pH 9.2 with ammonia water) and mobile phase B(acetonitrile).The flow rate was 1 mL·min-1 and the detective wavelength of DAD was set at 220 nm.Results:The linear ranges of matrine,oxymatrin,sophocarpine,oxysophocarpine and sophoridine were 0.1575-2.205 μg(r=0.9999),0.8985-12.58 μg(r=0.9999),0.1360-1.904 μg(r=0.9999),0.4398-6.153 μg(r=0.9998),and 0.2092-2.926 μg(r=0.9998),respectively;The recoveries(n=3) were 101.0%-102.5%(RSD≤2.1%),100.8%-101.6%(RSD≤2.8%),102.3%-103.6%(RSD≤1.2%),102.2%-103.4%(RSD≤1.1%),and 102.4%-103.3%(RSD≤2.9%),respectively.Conclusion:The HPLC method shows a good precision and accuracy.The total contents of matrine and oxymatrine in both Kushen's rhizomes and roots are not less than 1.0%,which meet thestandardsin ChP(Vol.I)2010 Ed.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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