Title: Study on HPLC-MS Total Ionic Chromatographic Fingerprints of Radix astragali
Abstract:OBJECTIVE To establish an evaluation method of Radix astragali by HPLC-MS total ionic chromatographic fingerprints.METHODS The conditions of experiment were confirmed and the validation of the method ...OBJECTIVE To establish an evaluation method of Radix astragali by HPLC-MS total ionic chromatographic fingerprints.METHODS The conditions of experiment were confirmed and the validation of the method was also tested.The separation was performed on an agilent zorbax SB C_(l8) column(4.6 mm×250 mm,5 μm) with a gradient elution.The mobile phase consisted of A(ACN) and B(H_(2)O).The gradient elution,flow speed was 1.0 mL·min~(-1).The temperature of column was room temperature,inject volume was 10 μL.The atmosphere pressure ionization-electrospray was used with negative scan mode.The flow rate drying gas(N_(2)) was 9.0 L·min~(-1),nebulizer pressure was 206.7 kPa,drying gas temperature was 350 ℃, fragment voltage was 110 V,capillary voltage was 3 500 V.Radix astragali was extracted by ultrasonic method for 30 min with methanol.The chromatographic fingerprints were analyzed with similarity evaluation system for chromatographic fingerprint of TCM(A edition) that issued by committee of national pharmacopoeia.RESULTS HPLC-MS total ionic chromatographic fingerprints of Radix astragali showed good stability,precision and reproducibility.The RSDs of relative retention of times and peak areas were less than 4.5%,13 common peaks were demarcated and the results of similarity calculation were more than 0.94.CONCLUSION The study is helpful for the quality control of Radix astragali,also can effectively distinguish Radix astragali from other samples.Read More
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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