Title: Fingerprint Analysis of Platycodonis Radix by HPLC-ELSD
Abstract:Objective: To establish a method of fingerprint analysis for Platycodonis Radix by high performance liquid chromatography-evaporative light-scattering detector(HPLC-ELSD).Method: HPLC was performed on...Objective: To establish a method of fingerprint analysis for Platycodonis Radix by high performance liquid chromatography-evaporative light-scattering detector(HPLC-ELSD).Method: HPLC was performed on a Welch Materials XB-C18 analytical column(4.6 mm × 250 mm,5 μm) with gradient elution of acetonitrile-0.1% formic acid at the flow rate of 0.8 mL·min-1 with ELSD detector.The column temperature was maintained at 30 ℃,the temperature of drift tube was set at 50 ℃,and the gas flow(N2) was set at 1.2 L·min-1.Result: The HPLC-ELSD chromatographic fingerprint of chemical constituents was established from the 12 batches of Platycodonis Radix and it had 16 characteristic common peaks.Conclusion: The developed method is repeatable and stable,which is helpful to control the quality of Platycodonis Radix.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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