Title: Effects of low-dose LPS pretreatment on LPS-induced intra-uterine fetal death and preterm labor
Abstract:Objective To investigate the effects of low-dose Lipopolysaccharide(LPS) pretreatment on LPS-induced intra-uterine fetal death(IUFD) and preterm labor.Methods The present study consisted of two separa...Objective To investigate the effects of low-dose Lipopolysaccharide(LPS) pretreatment on LPS-induced intra-uterine fetal death(IUFD) and preterm labor.Methods The present study consisted of two separate experiments.Experiment 1,The pregnant mice were divided into 4 groups randomly.In LPS group,the pregnant mice received an intraperitoneal(i.p.) injection of LPS(120 μg/kg) on gestational day 15(GD15).The control mice received saline(control group).In LPS 4 h+LPS group,the pregnant mice were pretreated with low-dose LPS(10 μg/kg) 4 h before high-dose LPS(120 μg/kg) treatment.In LPS 24 h+LPS group,the pregnant mice were pretreated with low-dose LPS(10 μg/kg)24 h before high-dose LPS(120 μg/kg) administration.All animals were observed closely for evidence of preterm labor and delivery.The remaining pregnant mice were sacrificed on GD18.For each litter,the number of live fetuses,dead fetuses,embryo resorption,and implantation sites were counted.Experiment 2,All pregnant mice were divided into 4 groups randomly by using the same methods described above.12 pregnant mice each group were sacrificed 1.5 h after high-dose LPS treatment.Maternal serum and amniotic fluid were harvested for measurement of TNF-α and IL-10.Twelve pregnant mice each group were sacrificed 6 h after high-dose LPS administration.Placentas were collected for measurement of glutathione(GSH) and malondialdehyde(MDA).Maternal serum and amniotic fluid were harvested for measurement of nitrite plus nitrate.Results We found that high-dose LPS-induced IUFD was obviously attenuated when the pregnant mice were pretreated with low-dose LPS 24 h before high-dose LPS treatment.Consistent with its protective effect,when administrated 24 h before high-dose LPS,low-dose LPS pretreatment obviously inhibited the releases of TNF-α in maternal serum and amniotic fluid.Low-dose LPS pretreatment 24 h before high-dose LPS significantly attenuated LPS-induced placental lipid peroxidation and GSH depletion.However,when administrated 4 h before high-dose LPS, low-dose LPS pretreatment had no effect on LPS-induced release of TNF-α in maternal serum and amniotic fluid,worsened LPS-induced placental lipid peroxidation and GSH depletion,and increased nitric oxide production in maternal serum and amniotic fluid.Correspondingly,low-dose LPS pretreatment 4 h before high-dose LPS aggravated LPS-induced IUFD.Conclusion The effects of low-dose LPS pretreatment on LPS-induced developmental toxicity depends on the interval between two doses of LPS.When administrated 24 h before high-dose LPS,low-dose LPS pretreatment protects mice against LPS-induced IUFD via counteracting the releases of TNF-α and oxidative stress,whereas low-dose LPS pretreatment 4 h before high-dose LPS aggravated LPS-induced developmental toxicity.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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