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Title: $Preventive and therapeutic effect of insulin on lipopolysaccharide-induced acute lung injury in rabbits
Abstract: AIM:To observe the role of insulin in the prevention and treatment of lipopolysaccharide(LPS)-induced acute lung injury(ALI)in rabbits and to further explore the underlying mechanism by which insulin exerts protective effects on LPS-induced ALI.METHODS:New Zealand white rabbits were divided randomly into four groups:saline control group,LPS group,insulin+LPS group and LPS+insulin group.The animal model of ALI was established by intratracheal administration of LPS 0.5 mg/(ml·kg).Microinjection pump was used to inject solution through ear-edge vein.NS group and LPS group received normal saline for 4 hours.Insulin+LPS group received insulin mixed liquor 0.5 hour before intratracheal administration of instilled LPS.LPS+insulin group received insulin mixed liquor 0.5 hour after intratracheal administration of instilled LPS.The insulin mixed liquor was given continuously for 4 hours.During that period,the mean carotid arterial pressure(mCAP),the glucose of venous blood and the K+ concentration of arterial blood were monitored.After experiment,the histological changes of lungs were observed and the left lung wet-to-dry(W/D)weight ratios were evaluated.SOD and MDA in lung homogenate were also measured.RESULITS:mCAP declined persistently(8.09±1.12)kPa and the contents of glucose of venous blood persistently ascended(12.8±5.6)mmol/L in LPS group.But insulin kept mCAP at higher levels(P0.05).Histological studied showed that there were congestion,edema and the sequestration of inflammatory cells in lung tissues in LPS group,but lung injury was significantly alleviated in insulin+LPS and LPS+insulin groups.The W/D and the content of MDA highly increased and the activity of SOD decreased(P0.05)in LPS group.Insulin inhibited all the increased parameters and upgraded the activity of SOD(P0.05).CONCLUSION:Insulin prevents and relieves LPS-induced rabbit ALI,which may be related with the inhibition of oxidative stress.