Title: Protective effect of ischemic preconditioning on rat small-for-size liver graft and its mechanism
Abstract: Objective To investigate the effect of ischemic preconditioning on rat small-for-size liver graft and explore its mechanism. Methods A total of 120 SD rats were randomized into the non-warm ischemia group (NWI, n=40), warm ischemia group (WI, n=40) and ischemic preconditioning group (IPC, n=40). The model of rat small-for-size liver transplantation was established by two-cuff technique. The serum levels of ALT and AST were determined by the automatic biochemical analyzer 1d before and 1, 2, 3 and 5 d after the operation. Hepatic tissue (0. 5 g) was procured before perfusion and 0. 5, 1, 2 and 3 h after transplantation in NWI group, before warm ischemin and 0. 5, 1,2, and 3 h after transplantation in WI group, before and after IPC and 0. 5, 1, 2 and 3 h after transplantation in IPC group. Their NO density was determined with nitrose reduction. Results IPC decreased serum levels of AST and ALT after rat small-for-size liver transplantation, increased hepatic NO density in the early stage but reduced it in the late stage after reperfusion. Conclusions NO might play dual role in the ischemia and reperfusion injury of the rat liver. IPC can protect rat small-for-size liver graft from ischemia and reperfusion injury. The mechanism of which might be that IPC promotes NO synthesis of the liver graft in the early stage after reperfusion and improves hepatic microcircula-tion. Meanwhile, it inhibits NO synthesis of the liver graft in the late stage after reperfusion and alleviates the injury mediated by excessive NO.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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