Title: Protective effect of ischemic preconditioning on ischemia reperfusion injury in reduced-size rat liver transplantation model
Abstract: Objective To investigate the protective effect of ischemic preconditioning(IPC) on ischemia reperfusion in reduced-size liver transplantation model and explore its mechanism.Methods 36 adult male SD rats were randomly divided into two groups: 50% sized liver transplantation group(control group) and ischemic preconditioning group(IPC group).Serum ALT levels were examined at 2,6 and 24 h postoperatively.24 h after reperfusion,the histopathological changes,the content of MDA and SOD in liver grafts were detected.The myeloperoxidase(MPO) activity was assayed to reveal neutrophil infiltration into the grafts,and the TNF-α levels were determined with enzyme-linked immunosorbent assay(ELISA).Results Compared with control group,serum ALT levels were significantly lower 6 and 24 h after reperfusion in IPC group(P0.01).Histopathological analysis showed disruption of lobular architecture,apparent hepatocelluar degeneration accompanied by focal necrosis;significant edema,congestion and inflammatory cell infiltration in periportal area 24 hours after reperfusion in the control group,whereas the injury was attenuated in IPC group.In IPC group,as compared to the control group,the MDA level significantly decreased while SOD level significantly decreased(P0.01).Likewise,hepatic TNF-α levels and MPO activity at 24 hours after reperfusion significantly decreased(P0.01).Conclusion IPC evidently ameliorated ischemia reperfusion injury in reduced-size liver grafts,and the protective mechanisms might be closely mediated in part by the enhancement of antioxidant ability,inhibition of lipid peroxidation,and down-regulation of inflammatory reaction.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot