Title: [Regulation of vitality and IL-10 receptor expression of human decidual stromal cells in early pregnancy by IL-10, IFN-gamma].
Abstract: The study is to investigate the effect of IL-10,IFN-γon viability and IL-10 receptor expression of human decidual stromal cells(DSC) in early pregnancy.The vitality of DSC was detected by MTF. IL-10R1 and IL-10R gene expression of DSC were detected after treated by different concentrations of IL-10,IFN-γat 15min,30min,45min,60min.The vitality of DSC was significantly enhanced by the higher dose of IL-10 (100-10ng/ml)(P<0.05),and there was no significant stimulative role induced by the lower dose of it(0.10-1ng/ml)(P>0.05).The viability of DSC was inhibited by the highest dose of IFN-γ(1000ng/ml)(P<0.01),on the contrary,its viability was stimulated by the lower dose of IFN-γ(10ng/ml)(P<0.05).IL-10R1 was highly expressed by IL-10 of 10ng/ml within 15rain,and significantly reduced at 30min,then disappeared within 45rain;while IL-10R1 expression was not induced by IL-10(lng/ml) of lower concentration throughout 60min;IL-10R1 was briefly and lowly expressed by IFN-γof 100ng/ml, which effect on DSC at 45rain;The expression of IL-10R1 was moderate level induced by IFN-γof 10ng/ml within 30min,and reduced at 45min,then disappeared at 60min.There was no significant difference of IL-10R2 expression (P>0.05)between treatment and not with the above-mentioned cytokines.IL-10,IFN-γmay play an important role of immune regulation in early pregnancy by influencing IL-10R1 expression and vitality of DSC.
Publication Year: 2006
Publication Date: 2006-04-01
Language: en
Type: article
Indexed In: ['pubmed']
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