Title: [Hydrogen peroxide upregulates interleukin-1beta-induced cyclooxygenase-2 expression in human pulmonary epithelial cells].
Abstract: To investigate the effect of hydrogen peroxide (H(2)O(2)) on interleukin-1beta (IL-1beta)-induced cyclooxygenase-2 (COX-2) expression in human pulmonary epithelial cells (HPEC).HPEC were stimulated by IL-1beta or H(2)O(2) or both of them, COX-2 mRNA expression was determined by reverse transcription-polymerase chain reaction (RT-PCR), and prostaglandin E(2) (PGE(2)) release was detected by enzyme-linked immunosorbent assay (ELISA). Cells that were not stimulated by any reagent were used as controls.(1) The relative amount of COX-2 mRNA expression induced by 1, 5 and 10 mg/L of IL-1beta [(143.1 +/- 7.2)%, (179.9 +/- 9.0)%, (190.0 +/- 9.5)%, respectively] was significantly higher than that in the control group (32.9 +/- 1.7)% (P < 0.05, respectively). (2) The concentration of PGE(2) released by HPEC treated with 5 and 10 mg/L of IL-1beta [(20.86 +/- 5.23) x 10(-6) g/L, (31.16 +/- 2.64) x 10(-6) g/L, respectively] was significantly higher than that in the control group (10.49 +/- 0.36) x 10(-6) g/L (P < 0.05, respectively). (3) The relative amount of COX-2 mRNA expression induced by 0.10, 0.25 and 0.50 mmol/L of H(2)O(2) in the presence of IL-1beta [(149.2 +/- 7.5)%, (189.6 +/- 9.5)%, (239.1 +/- 12.0)%, respectively] was significantly higher than that in the cells treated with IL-1beta alone (66.1 +/- 3.7)% or that in the control group (41.6 +/- 2.1)% (P < 0.05, respectively). (4) The concentration of PGE(2) released by HPEC treated with 0.10, 0.25 and 0.50 mmol/L of H(2)O(2) in the presence of IL-1beta [(27.01 +/- 5.16) x 10(-6) g/L, (32.79 +/- 3.01) x 10(-6) g/L, (41.13 +/- 3.41) x 10(-6) g/L, respectively] was significantly higher than that in the control group (10.49 +/- 0.36) x 10(-6) g/L (P < 0.05, respectively). The concentration of PGE(2) released by cells treated with 0.25 or 0.50 mmol/L of H(2)O(2) in the presence of IL-1beta was significantly higher than that in the cells treated with IL-1beta alone (20.86 +/- 5.23) x 10(-6) g/L (P < 0.05, respectively).Hydrogen peroxide upregulates IL-1beta-induced COX-2 expression in HPEC at transcriptional level.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
Indexed In: ['pubmed']
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Cited By Count: 2
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