Title: Study on enhanced resist to hypoxic/hypoglycemic condition by IL-32β in cervical carcinoma C33A cells
Abstract: Objective
To explore the enhancement effects and mechanisms of IL-32β on human cervical carcinoma cells C33A to hypoxic/hypoglycemic condition.
Methods
After cultured in hypoxia/hypoglycemic circumstance and normal circumstance for 20 hours respectively, the mRNA and protein expression of IL-32β in C33A cells were detected by real time-polymerase chain reaction (RT-PCR) and Western blotting respectively. Trypan blue stain was used to detect C33A cells viability in hypoxia/hypoglycemic circumstance and adding 10, 100, 500 ng/ml IL-32β circumstance. The xenografted tumor of nude mice was established by intraperitoneal injection, and their volumes were tested for a given time after injecting 0, 1.0 mg/kg IL-32β. siRNA was used to construct IL-32β knockdown cells and detect the expression of VEGF.
Results
Under the hypoxia/hypoglycemic circumstance, the expressions of IL-32β mRNA were (6.12±0.03) times of the normal circumstance (F=43.16, P<0.05), the expressions of IL-32β protein were (2.23±0.04) times of the normal circumstance (F=22.32, P<0.05). The C33A cells viability in hypoxia/hypoglycemic circumstance was (51.92±3.41)%, whereas, viability in 10 ng/ml IL-32β group was (55.23±3.92)% (F=14.25, P<0.05), viability in 100 ng/ml IL-32β group was (62.52±4.14)% (F=35.53, P<0.01), viability in 500 ng/ml IL-32β group was (69.14±2.45)% (F=56.28, P<0.01). After 28 days, the volume of xenografted tumor of 0 mg/kg IL-32β group was (578±64)mm3, and 1.0 mg/kg IL-32β group up to (1 402±142)mm3 (F=27.84, P<0.01). In addition, compared with control group, the expression of VEGF in IL-32β knockdown C33A cells was significantly decreased (F=36.85, P<0.05).
Conclusion
IL-32β can enhance the resistance to hypoxic/hypoglycemic condition of C33A cells, which is associated with the increase of VEGF.
Key words:
Uterine cervical neoplasms; Interleukins; Vascular endothelial growth factors; Hypoxic/hypoglycemic
Publication Year: 2015
Publication Date: 2015-11-08
Language: en
Type: article
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