Title: Development of a SCAR Marker Linked to Apple Spot Resistance Gene from a RAPD Marker
Abstract: A RAPD marker of apple spot resistance gene was converted into a SCAR marker successfully.Specific primers were designed according to the nucleotide sequence and tested in parents,bulks and in individuals of F1 progeny.Results showed that the primer pair could amplify a apple-spot-resistance-specific band of 470 bp.This SCAR marker was named as SCAR470.Meanwhile,the recombinant frequency is 4.69% based on amplification analysis in a segregation population.These results further confirmed the feasibility of this SCAR marker in marker-assisted selection(MAS) for apple spot.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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