Title: Identification of RAPD Marker Linked to Pollen Fertility Gene and Conversion to SCAR Marker in Peach
Abstract: In this study,a RAPD marker(OPW03-900) linked to pollen fertility gene was screened from 180 RAPD arbitrary primers in 52 F1 individuals from the cross 'Chongyanghong’×'Okuba’, pollen fertility/sterility pool was constructed respectively by using RAPD technique and BSA method. After repeating tests and checking up individuals of population, this marker could only be amplified in the pollen fertility individuals (except recombinants), the linkage distance was 5.80 cM with the pollen fertility/sterility locus. This band was collected and sequenced to synthesize a pair of SCAR primer which was used to amplify the individuals of the F1 progeny, the locus of the special band and the number of recombinants were the same as the result of RAPD amplification, the size of this marker was 906 bp,which was designated as SCW03-906, indicating the RAPD marker has been successfully converted into a SCAR marker which linked to pollen fertility gene in peach. This sequence was adopted by GenBank, the logging number was DQ659676.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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