Title: Experimental study on in vitro purgation of HL-60 cells by interferon combined with recombinant human tumor necrosis factor
Abstract: Objective To observe the effectiveness of alpha-interferon(α-IFN) combined with recombinant human tumor necrosis factor(rhTNF)on purgation HL-60 cells in vitro.Methods Different concentrations of α-IFN and rhTNF were mixed with HL-60 cells for 2 hours,then cultured it in vitro and calculated clearance rate.Results Combination of α-IFN and rhTNF showed a synergic elimination on HL-60 cells with a celarancer rate of above 90%,but no synergic inhibition on the normal bone marrow CFU-GM9 (inhibitory rate only 35%) was observed.Concentrations of both α-IFN and rhTNF were 100U/ml showed best results. Conclusion α-IFN combined with rhTNF can be used to remove the residual leukemic cells from autologous bone marrow.
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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