Title: The effects of MTX and rhTNF to cultured chondrosarcoma cell lines
Abstract: OBJECTIVE To study the effects of methotrexate(MTX) and recombinant human tumor necrosis factor(rhTNF) to cultured chondrosarcoma cell lines.METHODS Chondrosarcoma cells were undergone different contents MTX and rhTNF using the technique of cell culture.Four days later,cell morphology was observed by light microscope,growth states was studied by MTT method,Apoptosis was researched by fluorescein stain and flow cytometry.RESULTS The proliferation of chondrosarcoma cell was obviously restrained.The number of apoptosis cell of chondrosarcoma cell was significant increased(P0.01).These functions of MTX和rhTNF were enhanced following the increasing of drug's density.Each function of MTX和rhTNF can be enhanced on the condition of combined using.CONCLUSION MTX and rhTNF had the activity of anti-chondrosarcoma and had synergistic action.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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