Title: Construction of Co-expressing Plasmid of FMDV P1-2A Gene with Porcine IFN-γ Gene and Expression in BHK-21 Cells
Abstract: To construct the co-expressing plasmid of FMDV P1-2A gene with porcine IFN-γ gene and express in BHK-21 cells. A P1-2A gene of foot-and-mouth disease virus(FMDV) was amplified by PCR from the plasmid pGEM-P12A and subcloned into pBudCE-IFN vector for sequencing after purification and digestion. A recombinant plasmid pBudCE-IFN-P1-2A was constructed and transformed into BHK-21 cells with liposome for in vitro expression. 48 hours after transfection,the mRNAs and expressed proteins of P1-2A and IFN-γ were detected by RT-PCR and direct immunofluorescence assay,respectively. The results showed that P1-2A and IFN-γ were successfully transcripted and expressed in BHK-21 cells. It was proved that the recombinant plasmid pBudCE-IFN-P1-2A was successfully constructed and expressed in vitro,providing a solid basis for development of DNA vaccine against FMDV.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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