Title: Construction of recombinant eukaryotic expression plasmid of porcine IFN-gamma
Abstract: IFN-gamma(IFN-γ) gene of Landrace porcine was amplified by RT-PCR, and sequencing result was showed that the cloned gene had 100% homogeneity with the published sequence. The recombinant eukaryotic expression plasmid IFN-γ-pcDNA was constructed by inserting IFN-γ gene into pcDNA3.1(+).The recombinant plasmid was transfected into COS7 and the supernatant showed inhibition activity to porcine reproductive and respiratory syndrome virus (PRRSV).
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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