Title: Expression and detection of 3D gene of FMDV in recombinant baculovirus
Abstract: According to the 3D gene of FMDV,the primers of polymerase gene were designed,and the objective fragment was (1 410 bp) which was cloned into pMD18_T vector.Then the 3D gene was cloned into baculovirus expression vectors pFastBacⅠ,and the recombinant plasmid of pFastBac_3D was constructed.The plasmid were transformed to DH10 Bac competent cell,and was screened by three antibiotics and blue_white patch, the white colony was the recombinant Bacmid_3D.And then the bacmid_3D was transfected into the Sf9 insect cells,by three passaging the recombinant baculovirus was obtained.Finally,the expressed productions were detected by SDS_PAGE and Western_blotting,and the results showed that the polymerase(3D protein) was high_efficiently expressed in recombinant baculovirus.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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