Title: Effect of SAHA on TGF-β-induced Collagen Protein Expression in Human Embryonic Lung Fibroblast Cells
Abstract: Objective To investigate the effect of suberoylanilide hydroxamic acid(SAHA) on transforming growth factor-β1(TGF-β1)-induced collagen protein expression in human embryonic lung fibroblast cells(HELF).Methods HELF cells were cultured in vitro and divided into Control group(group A),SAHA group(group B),TGF-β group(group C) and SAHA groups(group D) according to the different purpose.PBS was used as negative control,and the cells were stimulated with 5 ng/mL of TGF-β for 24h.For group B,5mol/L SAHA was added.In group C,5 ng/mL of TGF-β was used.HELF cells in group D were treated with 5 ng/mL of TGF-β followed by SAHA at 0.5mol/L,2mol/L or 5mol/L for an additional 24h.Concentration of Hyfroxyprotein was determined by colorimetry,and the mRNA levels of I,Ⅲ procollagen in HELF cells were detected by RT-PCR.Results Hydroxyproline expression was very low in the control group,while the hydroxyproline content reached to(12.684±1.485) μg/mL after TGF-β1 treatment.When HELF cells were treated with 0.5 μM,2 μM or 5 μM SAHA,hydroxyproline content decreased with the increase of concentration of SAHA(P0.05).After 24h incubation of SAHA,mRNA expression of type I and type Ⅲ procollagen can be inhibited by stimulation of TGF-β1 in dose-dependent manner.Conclusions SAHA can inhibit TGF-β1-induced Hyfroxyprotein production,I and type Ⅲ procollagen expression,and this may be one of the anti-fibrosis mechanisms.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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