Title: Effect of AcSDKP on the collagen synthesis and expression in cultured rat cardiac fibroblasts mediated by transforming growth factor beta-1
Abstract: Objective To investigate whether AcSDKP can inhibit collagen synthesis in cultured rat cardiac fibroblasts mediated by transforming growth factor beta-1(TGF-β_1). Methods Neonatal rat cardiac fibroblasts were isolated. The synthesis of collagen was measured by 3H-proline incorporation assay. The expressions of type Ⅰ and Ⅲ collagen protein were detected by immunocytochemistry and Western blotting. Results 3H-proline content increased gradually (147.6±10.2, 229.2±16.4,427.0±40.6,454.8±26.1 CPM) and was 1.61, 2.50,4.66 and 4.97 times of control group(91.6±9.8 CPM) respectively in rat cardiac fibroblasts mediated by TGF-β_1 concentration of 1μg/L, 2.5μg/L, 5μg/L and 7.5μg/L. Compared with TGF-β_1 group (5μg/L), 3 H-proline content decreased gradually(378.8±6.4,292.8±14.4,130.6±17.6 and 230.6±19.4 CPM) and was 88.7%, 68.6%, 30.6% and 54.0% of TGF-β_1 group with increasing of AcSDKP concentration(10 -10 mol/L, 5×10 -10 mol/L, 10 -9 mol/L, 10 -8 mol/L). Compared with control group, the expression of collagen type Ⅰ and type Ⅲ in rat cardiac fibroblasts increased significantly in TGF-β1 group(5μg/L). Obsorbance values of collagen type Ⅰ and type Ⅲ in rat cardiac fibroblasts in TGF-β_1 group were respectively 1.36 and 2.12 times of control group by immunocytochemistry assay and 1.09 and 1.29 times of control group by Western blotting. However, the expression of collagen type Ⅰ and type Ⅲ in rat cardiac fibroblasts decreased significantly in AcSDKP group(10 -9 mol/L) compared with TGF-β_1 group(5μg/L). Obsorbance values of collagen type Ⅰ and type Ⅲ in rat cardiac fibroblasts in AcSDKP group were respectively 61.3% and 68.5% of TGF-β_1 group by immunohistochemistry assay and 83% and 54% of TGF-β_1 group by Western boltting. Conclusion AcSDKP can inhibit collagen synthesis and expression of type Ⅰ and Ⅲ collagen protein in cultured rat cardiac fibroblasts mediated by TGF-β_1, which is possibly related with the effect of AcSDKP anti-fibrosis.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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Cited By Count: 1
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