Title: Expression of nuclear factor-kappa B in hepatic tissue and its significance in rats after repeated and sustained +10Gz exposure
Abstract:Objective To observe the injury to liver cells, and to investigate the changes in nuclear factor-kappa B (NF-κB) in the liver tissue after exposure to positive ten acceleration (+10Gz). Methods Twenty...Objective To observe the injury to liver cells, and to investigate the changes in nuclear factor-kappa B (NF-κB) in the liver tissue after exposure to positive ten acceleration (+10Gz). Methods Twenty-four adult male Wistar rats were randomly assigned to four groups: blank control, exposure groups A, B, and C. The rats of three exposure groups were subjected to centrifugal force of +10Gz for 0.5, 24, and 48h respectively, which was carried out in an Air Force Institute of Aviation Medicine. Plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were determined. Liver tissue was harvested for immunohistochemistry, and Western blotting was performed for expression of NF-κB. Results After +10 Gz exposure, rats of the group A showed shaggy hair, apathetic to food, and laid still, while group B rats showed shaggy hair, took a little food with lowering of activity level. The rats in group C showed normal activities. Both the levels of serum AST and ALT were elevated, and they were higher in group B than that of groups A and C (P 0.05). Western blotting and immunohistochemical staining showed that the NF-κB expression levels were significantly higher in exposure groups than in control group (P 0.05). Immunohistochemical examination revealed NF-κBpositive products were localized predominantly in the hepatocytes, and also in the inflammatory cells and Kupffer cells. These products could be divided into three types: cytosolic type, karyotype, and nuclear-cytoplasm type, and they existed alone or in combination. Conclusion Exposure to +10Gz, especially in the early stage, acts as a stress to induce the activation of NF-κB, suggesting that NF-κB plays an important role in the cascade reactions and adaptation to the acceleration stress.
DOI: 10.11855/j.issn.0577-7402.2015.07.17Read More
Publication Year: 2015
Publication Date: 2015-01-07
Language: en
Type: article
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