Title: The Effect of Traditional Tibetan Tonic Prescription Basangmusuyou Pills on Proliferation Ability of Bone Marrow Cells in Mice Injured by Radiation and Chemistry
Abstract: Objective To research on the effect of traditional Tibetan tonic prescription Basangmusuyou pills on the bone marrow cells(BMC) proliferation ability in mice injured by radiation and chemistry and define the possible response cell groups,and to explore the pharmacodynamics mechanism of Basangmusuyou pills in stimulating peripheral hemogram recovery in these mice.Methods We used a flow cytometry and a colony forming assay in vitro to test the changes of stem cell antigen-1 positive(Sca-1+) cell proportion in BMC,the proportion of BMC proliferation cycle in every phase,and the early and late period hematopoietic progenitor colony forming unit of erythropoiesis(CFU-E,BFU-E),as well as the granlulocyte-macrophge,the megakaryocyte hematopoietic progenitor colony forming unit(CFU-GM and CFU-Meg) counts in mice injured by radiation and chemistry after gastric perfusion with Basangmusuyou pills(BSM group).Then,we carried out comparison among mice in the BSM group,mice with physiological saline gastric perfusion(PS group),and mice without any gastric perfusion(blank group).Results The S+G2/M phase proportion of BMC in BSM group was significantly higher than that in the PS group(P0.05) and the blank group(P0.01).The CFU-E,BFU-E,CFU-GM counts of BMC in BSM group were significantly higher than those in the PS group and the blank group(P0.05).Neither the CFU-Meg counts nor the Sca-1+ proportions of BMC in BSM group were significantly different from the PS group and the blank group(P0.05).Conclusion Basangmusuyou pills can stimulate BMC proliferating and the peripheral hemogram recovery,and the stimulation effect was different in different hematopoietic stages and line cells.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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