Title: Determination of the Content of Berberine Hydrochloride in Baitouweng Powder by HPLC
Abstract: To determine the content of berberine hydrochloride in Baitouweng powder and to provide evidence for its quality criteria,A HPLC method was established as follows:eclipse XDB-C18 column (4.6 mm×150 mm,5 μm);acetonitrile,0.05mol/L KH2PO4 =28∶72 as mobile phase,flow rate at 1.0 mL/min and detection wavelength was at 348 nm.The calibration curve of berberine hydrochloride was linear in the range of 0.05~0.50 μg with the correlation coefficient of 0.999 9.The average content of berberine hydrochloride in Baitouweng powder was 0.781%,and RSD=0.86%.This method is simple,accurate and repeatable,and can be used for the quality of Baitouweng powder.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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