Title: Effect of Huazhuo Jiedu Huoxue Recipe on cardiac enzyme and hemorheology in rats with acute myocardial ischemia
Abstract: Objective To study the effect of Huazhuo Jiedu Huoxue Recipe on cardiac enzyme and hemorheology in rats with acute myocardial ischemia.Methods Fifty Wistar male rats were randomly divided into five groups,namely control group,model group,isosorbide dinitrate group and Huazhuo Jiedu Huoxue Recipe high-and low-dose group(hereinafter referred to as the high-and low-dose group),n=10 in each group.The control group and model group were to be 10 mL/kg 0.9% sodium chloride injection,gavage.Isosorbide dinitrate group orally received suspension of 10 mL/kg isosorbide dinitrate to(from 2.5 mg/kg of isosorbide dinitrate added 10 mL 0.9% sodium chloride injection formulated).High-and low-dose group were to Huazhuo Jiedu Huoxue Recipe high dose(containing crude drug 4.70 g/mL),low-dose(containing crude drug 2.35 g/mL) 10 mL/kg orally once a day,for continuous 10 d.The first 10 d after the last administration 1 h,in addition to the control group,the rest of the group model of myocardial ischemia were produced with left anterior descending coronary artery ligation,only the surgical,and no ligation in control group.Administration was continued to 2 d after modeling.Blood from abdominal aorta of rats was taken to measure whole blood viscosity and blood hemorheology in each group,whole blood viscosity(high shear,low shear),plasma viscosity and hematocrit.Then the rats were decapitated,taking blood to measure creatine phosphokinase(CPK),aspartate aminotransferase(AST),lactate dehydrogenase(LDH).Results Hemorheology indexes and CPK,AST,LDH in model group and each treatment group were differ from those in control group(P0.05),modeling was success.There were significant differences between each treatment group and model group on whole blood viscosity(high shear),whole blood viscosity(low shear),CPK and LDH(P0.05).There were significant differences between high-dose group and model group on the plasma viscosity and hematocrit(P0.05).There was significant difference between low-dose group and model group on hematocrit(P0.05).There were significant differences between high-dose group and isosorbide dinitrate group on hemorheology indexes(P0.05).Conclusion Huazhuo Jiedu Huoxue Recipe can reduce the enzymes CPK,AST,LDH activity,improve myocardial ischemia hemorheology,has a protective effect on myocardial ischemia,its mechanism may be the expansion of the coronary arteries,improving blood circulation within the heart,increasing coronary blood flow,increasing myocardial oxygen supply.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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