Title: Effects of epimedium flavonoids injection on redox status in neonatal rat cardiomyocytes
Abstract: OBJECTIVE To explore the effects of EFI on early apoptosis and redox status in neonatal rat cardiomyocytes.METHODS Ventricular myocytes were isolated from neonatal rat and cultured.The oxidative stress model for neonatal rat cardiomyocytes was made by H2O2.Earlyapoptosis rates and ROS levels were detected by flow cytometer.Total anti-oxidative abilities were measured.The protein expressions of thioredoxin(Trx) and superoxide dismutase(SOD) were observed by western blotting.RESULTS After administrating 100 μmol·L-1 H2O2 for 12 h,the early apoptosis rates were enhanced significantly(P0.001),mean fluorescence intensity(MFI)was increased obviously(P0.01),total anti-oxidative ability was decreased remarkably(P0.01 or P0.05),Trx and SOD protein levels were reduced.EFI dose-dependently decreased early apoptosis rates and MFI,enhanced total anti-oxidative abilities,Trx and SOD protein levels were increased as well.Compared with H2O2 group,there were different significantly between the groups treated with 200 and 400 mg·L-1 EFI(P0.05 or P0.01).CONCLUSION H2O2 can induce neonatal rat cardiomyocytes to be in early apoptosis and in oxidative stress state.EFI can reduce the redox state in neonatal rat cardiomyocytes.It suggests that EFI can protect the oxidative injury in neonatal rat cardiaomyocytes induced by H2O2.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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