Title: The Effect of L-alanine on the Insulin Secretion of INS-1E Cells
Abstract: Objective: To explore the glucose-dependency of L-alanine induced insulin secretion in INS-1E cells.Methods: INS-1E cells were incubated in the RPMI 1640 for 2 days,and then the cells were incubated in the Krebs-Ringer buffer (KRB) with 0.1% BSA for 30 min.After preincubation,the cells were incubated for 60 min in the modified KRB containing glucose and alanine according to the protocol.After the incubation the medium was collected for the insulin assay.Results: Alanine at concentration of 0.1-20 mmol/L potentiated the glucose (16.7 mmol/L)-stimulated insulin secretion (GSIS) in a concentration dependent manner.Glucose at concentration of 1.1-25 mmol/L enhanced insulin secretion in a concentration dependent manner.The concentration of 10 mmol/L L-alanine with 1.1-3.3 mmol/L glucose did not show any effect on the insulin secretion,but 10 mmol/L L-alanine enhanced glucose (6.7-25 mmol/L)-induced insulin secretion.Conclusion: The present study shows that in the presence of 16.7 mmol/L glucose,L-alanine concentration-dependently enhanced GSIS of INS-1E cells.This effect is dependent on a certain level of glucose.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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