Title: Diphasic effect of leptin on insulin secretion of rat islets in vitro
Abstract: Objective To investigate the effects of different concentrations of leptin on insulin secretion of rat islets in vitro under different conditions. Methods Isolated rat islets were incubated in the absence or presence of recombinant rat leptin (0, 1, 5, 10, 15, 50 or 100 μg/L) for 10 min or 2h at either 5.6 mmol/L or 16.7 mmol/L glucose. After incubation, the insulin in supernatant was assayed by radioimmunoassay. Results At 5.6 mmol/L glucose, a 10 min incubation with 1 or 5 μg/L leptin stimulated insulin secretion from cultured islets, and a 2h incubation with 5 μg/L leptin also stimulated insulin secretion, but a 2h incubation with ≥50 μg/L leptin inhibited insulin secretion. At 16.7 mmol/L glucose, a 10 min incubation with ≥50 μg/L leptin inhibited insulin secretion, and a 2h incubation with ≥5 μg/L leptin also inhibited insulin secretion. Conclusion Recombinant leptin shows a diphasic effect on insulin secretion of rat islets in vitro, which can be influenced by several factors including leptin concentration, environmental glucose concentration and exposure time.
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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