Title: The Acute Dose-Dependent Effect and Glucose-Dependency of L-Alanine on the nsulin Secretion of Mouse slets
Abstract: To explore acute dose-dependent effect and glucose-dependency of L-alanine on the insulin secretion of mouse islets,female NMR mice were anaesthetized with pentobarbital,and collagenase technique was used to obtain pancreatic islets.The islets were handpicked under a stereomicroscope and kept in modified RPM 1640 overnight at an atmosphere of 95%humidified air/5%CO2.Then the islets were incubated in the Krebs-Ringer buffer(KRB) with 0.1%BSA for 30 minutes at 37℃.After preincubation,the islets were individually incubated at 37℃ for 60 minutes in the modified KRB 100ul containing glucose and alanine according to the protocol.After the incubation the medium was collected for insulin assay.Alanine at concentrations of 0.1~20mM potentiated the glucose(16.7mM) induced insulin secretion in a concentration dependent manner with a maximal effect obtained at 20mM.The effect of L-alnine disappeared with lower glucose concentration.The present study shows that L-alanine can enhance glucose-induced insulin secretion of mouse islets,the effect depends on the glucose concentration.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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