Title: Effects of High Concentration of Vitrification Cryoprotectant on Revitalization and Developmental Potentiality of Human Oocytes
Abstract: Objective: To explore the effect of the duration that oocytes exposed to high concentration of vitrification cryoprotectants on thier revitalization and developmental potentiality.Methods: Two hundred and ninety unfertilized mature oocytes from 79 patients who accepted in vitro fertilization(IVF) embryo transplantation treatment during January 2010 to May 2011 were freezed and defrosted.The oocytes were divided into two groups according to their time exposed to cryoprotectants: group A with 147 oocytes and exposing time ranged 10 seconds to 20 seconds;group B with 143 oocytes and exposing time ranged 40 seconds to 50 seconds.Revival rates,fertility rates,cleavage rates,and high quality embryo rates of the two groups were compared.Results: Revival rate in group A was significantly higher than that in group B(74.83% vs 63.64%,P0.05).No obvious difference was found in fertilization rate,cleavage rate or high-quality embryo rate between the two groups(69.09% vs 68.13%,71.05% vs 61.29% and 22.78% vs 31.57%,P0.05).Conclusions: Shortening the exposure time of oocytes to high concentration of vitrification cryoprotectants is helpful to revitalization of oocytes.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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