Title: Optimization of enzymatic preparation for chitooligosaccharides
Abstract: In order to prepare chitooligosaccharides with water-solubility and physiological activity,in this experiment chitosan was degraded by cellulase,measuring degradation effect with the index of reducing sugar concentration.In this paper,the Single factor and orthogonal experiments were conducted to obtain the optimal hydrolysis conditions.The results showed that the optimum conditions were as following: cellulase addition 1600U/g,temperature 55℃,reaction time 7h and pH 5.4.Under the terms of this optimum technics,the concentration of reducing sugar was determined to 2.52mmol/L.Then the products of hydrolysis was separated by ultra-filtration to obtain chitooligosaccharides whose molecular weight were less than 10KDa,with a production rate of 69.51%.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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