Title: The prime-boost strategy with mucosal DNA vaccine and killed oil-emulsified vaccine against H9 subtype of avian influenza virus
Abstract:In order to determine the synergistic immune efficacy of the DNA vaccine with the killed oil-emulsified vaccine of H9N2 subtype avian influenza virus(AIV),the mucosal DNA vaccine named SL7207(pmcDNA-H...In order to determine the synergistic immune efficacy of the DNA vaccine with the killed oil-emulsified vaccine of H9N2 subtype avian influenza virus(AIV),the mucosal DNA vaccine named SL7207(pmcDNA-HA)was construsted,and the immunogenicity of the prime-boost strategy with the DNA vaccine and killed oil-emulsified vaccine was evaluated in chicken model.The hemagglutinin(HA)gene from the H9N2 subtype AIV was amplified with the RT-PCR and sub-cloned into vector pmcDNA3.1+.The recombinant plasmid was transformed into the attenuated Salmonella typhimurium SL7207,and the positive recombinant was designated as SL7207(pmcDNA3.1-HA).One-day-old commercial ISA brown chickens were immunized with SL7207(pmcDNA3.1-HA)plus killed oil-emulsified vaccine of AIV,SL7207(pmcDNA3.1-HA),killed oil-emulsified vaccine of AIV,SL7207(pmcDNA3.1),and PBS.It was shown that the intestinal mucosal antibody titers in combined inoculation and SL7207(pmcDNA3.1-HA)groups were significantly higher than those of other groups(P0.05).The result of protective immunity showed both the group of combined inoculation and SL7207(pmcDNA3.1-HA)providing significantly protection compared with the negative control groups(P0.05),and the group of combined inoculation obtaining 100% protection.In conclusion,the mucosal DNA vaccine of H9N2 subtype AIV was successfully constructed,and the mucosal DNA vaccine plus killed oil-emulsified vaccine has good synergistic effect of protection.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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Cited By Count: 1
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