Title: Determination of The Damage of Three Pesticides on DNA by Absorption Spectrophotometry and Micronucleus Test
Abstract: To study the effect of chlorpyrifos,fenvalerate and malathion on the calf thymus DNA and Vicia faba root tip cells,DNA absorption spectrophotometry and micronucleus test were employed in the experiment.Formation of DNA adducts may serve as an indicator of DNA damager.Detection of binding of chlorpyrifos,fenvalerate and malathion with calf thymus DNA was based on a shift of DNA absorption spectrum.The results showed that the three pesticides resulted in the displacement of absorption peak(207 nm)moving to long wavelength for calf thymus DNA.The displace activities gradually increased along with the increase in concentration.The three pesticides also caused hypochromic effects of the DNA.These phenomenas indicated that pesticides were bound with DNA and had caused the potential trauma to DNA.If these DNA adducts are not repaired or misrepaired before cell duplication,the gene mutation and chemical damage would happen.In the micronuclei test,the results showed that with a certain concentration,micronuclei rate increased as the concentrations increased.At the low dose(0.1 μg·mL-1),there were no effect on the micronuclei rate,compared with the negative controlled group(P0.05).But there were adverse effects when the dose increased,and the difference was significant as compared with the negative controlled group(P0.01).These three pesticides with a certain concentration had significant effects on cytogenetical toxicity of Vicia faba root tip cells.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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