Title: ISSR Analysis of Genetic Diversity of Two Populations of Macrobrachium nipponense in Jiangsu Province
Abstract: Inter-simple sequence repeat (ISSR) was used to detect the genetic variation of two geographical populations of Macrobrachium nipponense. The individuals of M. nipponense were collected from the Changjiang River and Taihu Lake. Six primers, which were sifted from all 77 ISSR primers, produced 44 polymorphic markers from two populations. The proportions of polymorphic loci of Changjiang and Taihu populations were all 63. 44%, and Shannon's Infor-mation indexes were respectively 0. 348 0 and 0. 346 5. These results showed that the genetic diversity indexes of the two populations were at the same level. Shannon's Information index and AMOVA analysis indicated that genetic variation of M. nipponense was derived mainly from individuals within populations and had no significant genetic divergence between populations. The UPGMA tree based on ISSR data supported this result and no distinct variation was de-tected. The results of ISSR analysis indicated that two populations of M. nipponense presented lower genetic diversity; and there was a relatively normal level of genetic variation and some significant genetic differentiation between two populations.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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