Title: Optimization of culture medium and fermentation conditions for the production of antagonistic substances by Actinomycetes 327
Abstract: The culture medium and fermentation conditions were investigated for improving the production of antagonistic substances by Actinomycetes 327# in shaking flasks.By single factor and orthogonal experiment,the optimal fermentation medium of 327# were obtained as follows: soybean flour 0.5%,(NH4)2SO4 1.5%,glucose 2.5%,NaCl 0.6%,CaCO3 0.6%.The optimal fermentation conditions were as follows: culture temperature 30℃,initial pH 7.0,culture volume 15 ml in 250 mL shaking flask.Under these conditions,the inhibition zone diameter of 327# fermentation filtrate reached 22.4±0.4 mm on the test plates of Penicillium after 3 d incubation.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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