Title: Fermentation Conditions of Antagonistic Strain XL12 against Alternaria alternate
Abstract: In order to increase the quantity of antifungal protein from Bacillus subtilis XL12,the strain was cultured under different conditions.The optimization of fermentation factors,such as the C/N proportion of basic medium,inoculation amount,initial pH,fermentation temperature and fermentation time,was made by single factor experiments.The result showed that the optimal fermentation condition was initial pH 6.95~7.05 and shaking flask in 220 r·min-1speed,fermentation temperature was 30~35℃ for 72 hours,premise of which the C/N ration is 30∶1,inoculation amount is 0.5%,under the conditions,the most quantity of antifungal protein was obtained by the 70%(NH4)2SO4salting out supernatant fluid of strain XL12 fermentation.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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