Title: Synergistic Inhibition of Evodiamine Combined with RO3306 on Human Hepatocarcinoma Cell Line HepG2
Abstract: Objective To investigate the proliferation inhibition and the apoptosis-induction effect of evodiamine(EVO) combined with RO3306,a specific CDK1 inhibitor,on human hepatocarcinoma cell line HepG2,and to study whether the effects are synergistic.Methods Methyl thiazolyl tetrazolium(MTT) assay,and propidium iodide(PI) staining associated with flow cytometry(FCM) assay were used to observe the occurrence of an irreversible apoptosis induced by EVO.MTT assay,colony-forming assay and FCM assay were used to investigate the synergistic action of EVO combined with RO3306.The evaluation criteria of synergistic action were q1.15.Results The results of MTT assay showed that a higher inhibition rate for HepG2 was presented when HepG2 cells were treated with EVO for 16 h and then cultured for another 12 h with EVO removed.The results of FCM assay showed that M-arrest occurred when HepG2 cells were treated with EVO for about 12 h after cell synchronization,and M-slippage occurred when HepG2 cells were treated with EVO for about 20 h.Results of MTT assay showed that the inhibitive rates in 2 μmol/L EVO group,2 μmol/L RO3306 group and the combination group were 33.64%,6.09% and 52.74%,respectively,and q=1.32.Results of colony-forming assay showed the inhibitive rates in 1 μmol/L EVO group,2 μmol/L RO3306 group and the combination group were 48.21%,11.13% and 78.13%,respectively,and q=1.83;the inhibitive rates in 2 μmol/L EVO group,2 μmol/L RO3306 group and the combination group were 9.75%,66.80% and 91.20%,respectively,and q=1.3.Results of FCM assay showed that the inhibitive rates in 2 μmol/L EVO group,2 μmol/L RO3306 group,and the combination group were 23.7%,6.6% and 44.8%,respectively,and q=1.31.Conclusion M-arrest of HepG2 occurs after treatment with EVO for about 12 h,and the inconvertible apoptosis occurs after treatment with EVO for about 20 hours.It is indicated that EVO combined with RO3306 shows an obvious synergistic inhibition on HepG2.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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