Title: Effects of Rosa roxburghii Tratt extract and matrine on proliferation and apoptosis of human endothelial cells cultured with supernatant of gastric cancer cells.
Abstract: Objective To investigate the effects of Rosa roxburghii Tratt extract(CL) combined with matrine(MA) on the proliferation and apoptosis of the human endothelial cell cultured with supernatant derived from gastric cancer cells.Methods Human endothelial EVC-304 cells cultured with supernatant of human gastric carcinoma SGC-7901 cells were exposed to CL and/or MA.MTT was used to measure cell proliferation.The expression levels of Ki-67 and Bcl-2 mRNA were detected by RT-PCR,and the expression levels of Ki-67,Bcl-2 and Bax proteins were evaluated by Western blot.Results After treated with CL(10,20,40,80 and 160 mg/L,for 48 h),MTT assay revealed the inhibition rates were(15.1±2.1)%,(23.1±2.3)%,(34.1±3.3)%,(46.9±3.8)% and(68.8±2.9)%,respectively;after treated with matrine(15,30,60,120 and 240 mg/L,for 48 h),the inhibition rates were(20.8±1.3)%,(25.1±2.2)%,(40.9±1.1)%,(62.9±2.2)% and(77.2±1.9)%,respectively;after treated with CL combined with matrine [(10+15)mg/L,(20+30)mg/L,(40+60)mg/L,(80+120)mg/L and(160+240)mg/L,for 48 h],the inhibition rates were(33.8±2.1)%,(46.8±2.4)%,(84.2±2.0)%,(88.1±2.2)% and(94.8±0.9)%,respectively;showing a dose-dependent manner(P0.05).After treated with IC50 CL(75 mg/L),IC50 matrine(75 mg/L) and 1/2 IC50 CL combined with 1/2 IC50 matrine [(37.5+37.5)mg/L,48 h],MTT assay revealed the inhibition rates were(40.7±1.3)%,(46.2±1.2)% and(51.4±0.7)%,respectively(P0.05);media-effect principle showed a synergistic effect of CL combined with MA.Compared with those in CL or MA alone,the levels of Ki-67 and Bcl-2 mRNA/protein expression were significantly decreased in combination group(P0.05);the levels of Bax mRNA/protein expression were significantly increased in combination group(P0.05).Conclusion CL/MA can inhibit the proliferation of the human endothelial cell;when CL was combined with MA,these two drugs show a synergistic effect.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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