Title: Experimental study on apoptosis of human gastric carcinoma SGC-7901 cells induced by Trametes robiniophila
Abstract: Objective To study the effects of Tramets robinophila on the proliferation inhibition and apoptosis induction of human gastric carcinoma SGC-7901 cells,and explore the mechanism. Methods Human gastric carcinoma SGC-7901 cells were treated with 0,0.5,1,2,4,6 mg/mL Tramets robinophila and 10 μg/mL 5-fluorouracil(5-Fu),and the cell proliferation inhibition rates were determined by MTT assay 24 h and 48 h after treatment.Gastric carcinoma SGC-7901 cells were collected,DNA was isolated 48 h after treatment and was subjected to agarose gel electrophoresis.In addition,cell apoptosis rates were determined by flow cytometry,and RT-PCR was employed to detect the expression of survivin mRNA. Results It was demonstrated by MTT assay that the growth of SGC-7901 cells was inhibited by Tramets robinophila in a time-and dose-dependent manner(P0.05).The inhibition rate of the group of 6 mg/mL Tramets robinophila reached the peak 48 h after treatment,significantly different from that in the group of 10 μg/mL 5-Fu [(77.9±2.3)% vs(53.4±1.6)%](P0.05).It was revealed by flow cytometry that the cell apoptosis rate was the highest in the group of 4 mg/mL Tramets robinophila,and the early apoptosis rate was 33.2%,while the early apoptosis rate and late apoptosis rate were 6.3% and 19.9%,respectively for the group of 6 mg/mL Tramets robinophila.It was indicated by RT-PCR that the expression of survivin mRNA in SGC-7901 cells in the groups of Tramets robinophila was down-regulated. Conclusion Trametes robiniophila has inhibitory effects on SGC-7901 cell proliferation and can induce cell apoptosis,which may be related to the inhibition of the expression of survivin mRNA.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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Cited By Count: 1
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