Title: Expression of PITX1 mRNA and hTERT mRNA in non-small cell lung cancer tissues
Abstract: OBJECTIVE:To explore the expression level of PITX1 and hTERT gene mRNA expression in non-small cell lung cancer(NSCLC).METHODS: The reverse transcription polymerase chain reaction(RT-PCR) was used to detect the PITX1 and hTERT mRNA in 91 cases of non-small cell lung cancer and 21 cases of lung benign diseases tissues separately.RESULTS: The expression rate of PITX1 gene in non-small cell lung cancer tissues was 24.18%(22/91),which was significantly lower than that in benign lung tissue 66.67%(14/21),and the difference was statistically significant(χ2=14.12,P=0.000).hTERT gene overexpression in 72(79.12%) of 91 patients with non-small cell lung cancer,significantly higher than its normal expression in benign lung tissue,and the difference was statistically significant(χ2=46.52,P=0.000).The difference was statistically significant in the PITX1 gene mRNA expression in lymph node metastasis(P=0.004) and the degree of differentiation(high,moderately differentiated and poorly differentiated;P=0.030).hTERT gene mRNA overexpression in pathological type was statistically significant(P=0.003).There was correlation between PITX1 gene mRNA and hTERT mRNA expression(P=0.000).CONCLUSIONS: PITX1 gene expression may inhibit the over-expression of hTERT gene.mRNA expression of PITX1 and hTERT may be related to the occurrence and development of non-small cell lung cancer.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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