Publication Information

{'id': 'https://openalex.org/W2340166475', 'doi': None, 'title': '[Expression and purification of GST-Rta fusion protein from EB virus and preparation of the polyclonal antibody against GST-Rta].', 'display_name': '[Expression and purification of GST-Rta fusion protein from EB virus and preparation of the polyclonal antibody against GST-Rta].', 'publication_year': 2005, 'publication_date': '2005-09-01', 'ids': {'openalex': 'https://openalex.org/W2340166475', 'mag': '2340166475', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/16235532'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://europepmc.org/article/MED/16235532', 'pdf_url': None, 'source': None, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5052393012', 'display_name': 'Yongchun Xu', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I24185976', 'display_name': 'Sichuan University', 'ror': 'https://ror.org/011ashp19', 'country_code': 'CN', 'type': 'education', 'lineage': ['https://openalex.org/I24185976']}], 'countries': ['CN'], 'is_corresponding': False, 'raw_author_name': 'Yong-Chun Xu', 'raw_affiliation_strings': ['Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China)'], 'affiliations': [{'raw_affiliation_string': 'Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China)', 'institution_ids': ['https://openalex.org/I24185976']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5029610764', 'display_name': 'Weibo Liang', 'orcid': 'https://orcid.org/0000-0002-8487-3575'}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'Wei-Bo Liang', 'raw_affiliation_strings': [], 'affiliations': []}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5026616571', 'display_name': 'Hang Gou', 'orcid': None}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'Hang Gou', 'raw_affiliation_strings': [], 'affiliations': []}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5100462254', 'display_name': 'Wenjie Chen', 'orcid': 'https://orcid.org/0000-0001-9512-9664'}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'Wen-Jie Chen', 'raw_affiliation_strings': [], 'affiliations': []}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5104114160', 'display_name': 'Yinhua Zhu', 'orcid': 'https://orcid.org/0009-0002-2803-3579'}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'Yin-Hua Zhu', 'raw_affiliation_strings': [], 'affiliations': []}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5102172269', 'display_name': 'Jing Jia', 'orcid': None}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'Jing Jia', 'raw_affiliation_strings': [], 'affiliations': []}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5100351842', 'display_name': 'Lin Zhang', 'orcid': 'https://orcid.org/0000-0002-3767-0249'}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'Lin Zhang', 'raw_affiliation_strings': [], 'affiliations': []}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 0.0, 'has_fulltext': False, 'cited_by_count': 0, 'citation_normalized_percentile': {'value': 0.0, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 0, 'max': 61}, 'biblio': {'volume': '36', 'issue': '5', 'first_page': '665', 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10613', 'display_name': 'Gene Therapy Techniques and Applications', 'score': 0.9624, 'subfield': {'id': 'https://openalex.org/subfields/1311', 'display_name': 'Genetics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10613', 'display_name': 'Gene Therapy Techniques and Applications', 'score': 0.9624, 'subfield': {'id': 'https://openalex.org/subfields/1311', 'display_name': 'Genetics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11663', 'display_name': 'Recombinant Protein Production in Mammalian and Insect Cells', 'score': 0.9128, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/polyclonal-antibodies', 'display_name': 'Polyclonal antibodies', 'score': 0.93183136}, {'id': 'https://openalex.org/keywords/sepharose', 'display_name': 'Sepharose', 'score': 0.5939297}, {'id': 'https://openalex.org/keywords/transgene-expression', 'display_name': 'Transgene Expression', 'score': 0.487595}], 'concepts': [{'id': 'https://openalex.org/C956191', 'wikidata': 'https://www.wikidata.org/wiki/Q930144', 'display_name': 'Polyclonal antibodies', 'level': 3, 'score': 0.93183136}, {'id': 'https://openalex.org/C123894998', 'wikidata': 'https://www.wikidata.org/wiki/Q425158', 'display_name': 'Fusion protein', 'level': 4, 'score': 0.8565067}, {'id': 'https://openalex.org/C40767141', 'wikidata': 'https://www.wikidata.org/wiki/Q285697', 'display_name': 'Recombinant DNA', 'level': 3, 'score': 0.7711396}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.7474244}, {'id': 'https://openalex.org/C179247698', 'wikidata': 'https://www.wikidata.org/wiki/Q382799', 'display_name': 'Affinity chromatography', 'level': 3, 'score': 0.7432157}, {'id': 'https://openalex.org/C2776415932', 'wikidata': 'https://www.wikidata.org/wiki/Q48743', 'display_name': 'Western blot', 'level': 3, 'score': 0.6611531}, {'id': 'https://openalex.org/C2779881004', 'wikidata': 'https://www.wikidata.org/wiki/Q7451876', 'display_name': 'Sepharose', 'level': 3, 'score': 0.5939297}, {'id': 'https://openalex.org/C170344550', 'wikidata': 'https://www.wikidata.org/wiki/Q311359', 'display_name': 'lac operon', 'level': 4, 'score': 0.56807256}, {'id': 'https://openalex.org/C159654299', 'wikidata': 'https://www.wikidata.org/wiki/Q79460', 'display_name': 'Antibody', 'level': 2, 'score': 0.5444826}, {'id': 'https://openalex.org/C2522874641', 'wikidata': 'https://www.wikidata.org/wiki/Q808', 'display_name': 'Virus', 'level': 2, 'score': 0.44038653}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.43601033}, {'id': 'https://openalex.org/C547475151', 'wikidata': 'https://www.wikidata.org/wiki/Q25419', 'display_name': 'Escherichia coli', 'level': 3, 'score': 0.4200891}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.39313814}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.2550906}, {'id': 'https://openalex.org/C159047783', 'wikidata': 'https://www.wikidata.org/wiki/Q7215', 'display_name': 'Virology', 'level': 1, 'score': 0.24491495}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.13076296}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.06851545}, {'id': 'https://openalex.org/C203014093', 'wikidata': 'https://www.wikidata.org/wiki/Q101929', 'display_name': 'Immunology', 'level': 1, 'score': 0.056414098}], 'mesh': [{'descriptor_ui': 'D005982', 'descriptor_name': 'Glutathione Transferase', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D017874', 'descriptor_name': 'Immediate-Early Proteins', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': True}, {'descriptor_ui': 'D015534', 'descriptor_name': 'Trans-Activators', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D014764', 'descriptor_name': 'Viral Proteins', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D000818', 'descriptor_name': 'Animals', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D000911', 'descriptor_name': 'Antibodies, Monoclonal', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D000911', 'descriptor_name': 'Antibodies, Monoclonal', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': False}, {'descriptor_ui': 'D020031', 'descriptor_name': 'Epstein-Barr Virus Infections', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D020031', 'descriptor_name': 'Epstein-Barr Virus Infections', 'qualifier_ui': 'Q000821', 'qualifier_name': 'virology', 'is_major_topic': False}, {'descriptor_ui': 'D005982', 'descriptor_name': 'Glutathione Transferase', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005982', 'descriptor_name': 'Glutathione Transferase', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': False}, {'descriptor_ui': 'D017874', 'descriptor_name': 'Immediate-Early Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017874', 'descriptor_name': 'Immediate-Early Proteins', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': False}, {'descriptor_ui': 'D008297', 'descriptor_name': 'Male', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011817', 'descriptor_name': 'Rabbits', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D051381', 'descriptor_name': 'Rats', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': False}, {'descriptor_ui': 'D015534', 'descriptor_name': 'Trans-Activators', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D015534', 'descriptor_name': 'Trans-Activators', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': False}, {'descriptor_ui': 'D014764', 'descriptor_name': 'Viral Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014764', 'descriptor_name': 'Viral Proteins', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://europepmc.org/article/MED/16235532', 'pdf_url': None, 'source': None, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/16235532', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [{'score': 0.64, 'id': 'https://metadata.un.org/sdg/3', 'display_name': 'Good health and well-being'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 0, 'referenced_works': [], 'related_works': ['https://openalex.org/W66749156', 'https://openalex.org/W3032815285', 'https://openalex.org/W2393364397', 'https://openalex.org/W2392020358', 'https://openalex.org/W2388821692', 'https://openalex.org/W2388255078', 'https://openalex.org/W2379756016', 'https://openalex.org/W2378010885', 'https://openalex.org/W2376844583', 'https://openalex.org/W2375597396', 'https://openalex.org/W2373856890', 'https://openalex.org/W2372477160', 'https://openalex.org/W2369020248', 'https://openalex.org/W2368129263', 'https://openalex.org/W2366226848', 'https://openalex.org/W2362612214', 'https://openalex.org/W2360779905', 'https://openalex.org/W2356428755', 'https://openalex.org/W2353518111', 'https://openalex.org/W2353199258'], 'abstract_inverted_index': {'OBJECTIVE': [0], 'To': [1], 'obtain': [2], 'the': [3, 38, 91, 94, 113, 132, 143], 'recombinant': [4, 39], 'fusion': [5, 136], 'proteins': [6, 40, 57, 67, 87, 96, 137], 'GST-Rta185': [7], 'and': [8, 13, 26, 43, 73, 93, 108, 118, 141], 'GST-Rta150': [9], 'from': [10, 60, 138], 'EB': [11, 139, 148], 'virus': [12, 140, 149], 'prepare': [14], 'two': [15], 'Rta': [16, 135], 'protein': [17], 'specific': [18], 'polyclonal': [19, 114], 'antibodies,': [20], 'respectively.': [21], 'METHODS': [22], 'Plasmids': [23], 'pGEX-R1501,': [24], 'pGEX-R1851': [25], 'pGEX-5X-3': [27], 'were': [28, 46, 58, 68, 75, 88, 97, 120], 'separately': [29], 'transformed': [30], 'into': [31], 'Escherichia': [32], 'coli': [33], 'BL21': [34], '(DE3).': [35], 'Expressions': [36], 'of': [37, 83, 85, 147], 'R150-GST,': [41], 'R185-GST': [42], 'free': [44], 'GST': [45], 'induced': [47], 'by': [48, 99], '0.1': [49], 'mmol/L': [50], 'IPTG': [51], 'in': [52, 90], 'LB': [53], 'medium.': [54], 'The': [55, 123], 'expressed': [56], 'purified': [59, 95], 'lysates': [61, 92], 'with': [62, 70, 102], 'Glutathione': [63, 103], 'Sepharose': [64, 104], '4B.': [65, 105], 'Purified': [66], 'mixed': [69], "Freund's": [71], 'adjuvant': [72], 'then': [74], 'used': [76], 'to': [77], 'immunize': [78], 'rabbits.': [79], 'RESULTS': [80], 'High': [81], 'levels': [82], 'expression': [84], 'target': [86], 'detected': [89], 'obtained': [98], 'affinity': [100], 'chromatography': [101], 'Western': [106], 'blot': [107], 'ELISA': [109], 'analysis': [110], 'suggested': [111], 'that': [112], 'antibodies': [115], 'against': [116], 'GST-R185': [117], 'GST-R150': [119], 'specific.': [121], 'CONCLUSION': [122], 'antiserums': [124], 'have': [125], 'good': [126], 'specificity.': [127], 'They': [128], 'are': [129], 'important': [130], 'for': [131, 142], 'research': [133], 'on': [134], 'diagnosis': [144], 'or': [145], 'treatment': [146], 'associated': [150], 'diseases.': [151]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2340166475', 'counts_by_year': [], 'updated_date': '2024-09-19T03:04:34.880891', 'created_date': '2016-06-24'}