Publication Information

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'https://openalex.org/W3032815285', 'https://openalex.org/W3031746902', 'https://openalex.org/W3029711171', 'https://openalex.org/W3002398566', 'https://openalex.org/W2875310867', 'https://openalex.org/W2583363840', 'https://openalex.org/W2415911233', 'https://openalex.org/W2414179442', 'https://openalex.org/W2385727787', 'https://openalex.org/W2371728522', 'https://openalex.org/W2369528465', 'https://openalex.org/W2368129263', 'https://openalex.org/W2357578130', 'https://openalex.org/W2348991363', 'https://openalex.org/W2340166475', 'https://openalex.org/W2181706904'], 'abstract_inverted_index': {'Objective': [0], 'To': [1], 'express': [2], 'PA28γ': [3, 16, 78, 121], 'antigen': [4], 'as': [5], 'a': [6], 'GST-PA28γ': [7, 34, 106], 'fusion': [8, 35, 44, 83], 'protein': [9, 36, 45, 69, 84, 122], 'and': [10, 32, 70, 90, 109, 123, 133], 'prepared': [11], 'anti-PA28γ': [12, 124], 'antiserum.': [13, 72], 'Methods': [14], 'The': [15, 24, 43, 117], 'gene': [17], 'was': [18, 27, 37, 46, 63, 80, 85, 107], 'cloned': [19], 'into': [20, 29], 'the': [21, 33, 67, 77, 96, 104, 131], 'vector': [22], 'pGEX-4T-1.': [23], 'recombinant': [25, 59, 105], 'plasmid': [26], 'transferred': [28], 'E.coli': [30], 'BL-21-STAR(DE3)': [31], 'expressed': [38, 68], 'by': [39, 48], 'inducing': [40], 'with': [41, 58], 'IPTG.': [42], 'purified': [47], 'glutathione': [49], 'sepharose': [50], '4B': [51], 'affinity': [52], 'chromatography.': [53], 'BALB/c': [54], 'mice': [55], 'were': [56], 'immunized': [57], 'PA28γ,and': [60], 'western': [61], 'blot': [62, 101], 'used': [64], 'to': [65], 'detect': [66], 'polyclonal': [71, 125], 'Results': [73], 'DNA': [74], 'sequencing': [75], 'confirmed': [76], 'sequence': [79], 'correct.': [81], 'Expressed': [82], 'mainly': [86], 'in': [87, 114], 'soluble': [88], 'supernatant': [89], 'accounted': [91], 'for': [92], 'about': [93], '15%': [94], 'of': [95, 120, 136], 'total': [97], 'bacterial': [98], 'proteins.': [99], 'Western': [100], 'results': [102], 'demonstrated': [103], 'antigenic': [108], 'could': [110], 'induce': [111], 'antibody': [112], 'production': [113], 'mice.': [115], 'Conclusion': [116], 'successful': [118], 'preparation': [119], 'antiserum': [126], 'facilitates': [127], 'further': [128], 'studies': [129], 'on': [130], 'structure,functions': [132], 'protein-protein': [134], 'interaction': [135], 'PA28γ.': [137]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2362612214', 'counts_by_year': [], 'updated_date': '2024-09-19T13:03:37.912707', 'created_date': '2016-06-24'}