Title: Expression of hPLIC-2 and antibody preparation
Abstract: hPLIC-2 gene was amplified by PCR and inserted into pGEX-2T vector. The recombinant plasmid was transformed into host cell JM109. GST-hPLIC-2 fusion protein was expressed at a high level as a soluble form after being induced by IPTG at 27 ℃. The soluble fusion protein was purified by Glutathione Sephorase 4B. The polyclonal antibody was made after immunizing rabbit by Glutathione Sephorase 4B binding GST-hPLIC-2 and the titer of the antiserum was more than 1∶16 000. This antibody will play important role in the functional study of hPLIC-2 gene.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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