Title: HPLC Simultaneous Determination of Baicalin and Arctiin in Xuanlan Yanhouyan Granules
Abstract:Objective To develop an HPLC method for the determination of baicalin and arctiin in Xuanlan Yanhouyan granules. Methods Analysis was carried out on a SHIMADZU VP-ODS column( 150 mm × 4. 6 mm,5 μm) wi...Objective To develop an HPLC method for the determination of baicalin and arctiin in Xuanlan Yanhouyan granules. Methods Analysis was carried out on a SHIMADZU VP-ODS column( 150 mm × 4. 6 mm,5 μm) with methanol-0. 1% phosphoric acid as the mobile phase,detectied at 280 nm. The column temperature was30 ℃ and the flow rate was 1. 0 ml·min- 1. Results The average recovery of baicalin was 99. 78%( RSD = 2. 28%)and the linear range was 25. 9- 259. 0 μg·ml- 1. The average recovery of arctiin was 100. 45%( RSD = 2. 26%)and the linear range was 5. 09- 50. 9 μg·ml- 1. Conclusion This method is simple and reproducible. It can be used for quality control of Xuanlan Yanhouyan granules.Read More
Publication Year: 2016
Publication Date: 2016-01-01
Language: en
Type: article
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