Abstract: This chapter discusses the radioimmunoassay for thromboxane B2 that reflect the concentration of its unstable, biologically active precursor, thromboxane A2. Various controls are advised when using a TxB2 radioimmunoassay. First, whenever possible, specific thromboxane synthase inhibitors, such as imidazole, alkylimidazoles, 9,11-iminoepoxyprosta-5,13-dienoic acid, should be included in the experimental design. These agents should suppress TxB2 concentrations in a dose-dependent fashion without suppressing the concentration of prostaglandins or other metabolites of arachidonic acid. Second, nonsteroidai anti-inflammatory agents, such as indomethacin, aspirin, or ibuprofen, should suppress TxB2 concentrations and the concentrations of other products of PGH synthase. It is important to recognize that TxB2 forms by the hydrolysis of its unstable precursor, TxA2. In samples containing albumin, TxA2 does not necessarily hydrolyze with a 100% yield of TxB2. Some TxA2 binds covalently to proteins or even biological membranes. To maximize the hydrolytic transformation of TxA2 into TxB2, it is advised to use a mild acid quench for samples where such covalent bonding is likely.
Publication Year: 1982
Publication Date: 1982-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 31
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