Title: In vitro rapid clonal propagation in static liquid medium and acclimatization of Piper solmsianum and P. tuberculatum
Abstract: An efficient and rapid tissue culture system is des cribed for multiplication of Piper solmsianum and P. tuberculatum, two important American species, th rough shoot tip and nodal explants cultures. Stem segments with 2-3 nodes (3-4 cm long), taken f rom eight to ten-month-olds in vitro plantlets, were placed on 0.6% agar gelled (G) and liquid (L) MS mineral salts plus vitamins, 3% sucrose and supplemented with 0.02 mg/L IAA + 0.02 mg/L GA 3 (G1 or L1), 0.02 mg/L IAA + 0.02 mg/L GA 3+ 0.02 mg/L BAP (G2 or L2) or 0.02 mg/L IAA + 0.02 mg /L GA 3+ 0.05 mg/L BAP (G3 or L3).The best shoot elongation, shoot proliferation and rooting rof stem segments was observed in liquid culture medium (L3). An average of 9.1±0.9 cm shoot elongation, 2.4±0.5 shoot proliferation, and 5.5±0.8 nodes formation for P. solmsianum, and 8.6± 0.7 cm shoot elongation, 2.1±0.3 shoot proliferation, and 6.7±0.6 nodes formation for P. t uberculatum were observed after 120 days of culture. For acclimatization, elongated shoots were separated and rooted in MS medium supplemented with 0.02 mg/L IAA + 0.02 mg/L GA 3. Plantlets, thus developed were established in several potting mixtures with 30 to 100% of surviva l rate in both species.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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Cited By Count: 2
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