Title: Globe artichoke plants obtained from shoot apices through rapid in vitro micropropagation
Abstract: A rapid method of in vitro propagation for globe artichoke (Cynara scolimus L.) is described. Shoot apices and subterminal stem segments were cultured on modified Murashige and Skoog (1962) medium (MS) with NaH2PO4 (50 mg/l), m-inositol (100 mg/l), L-tyrosine (100 mg/l), adenine (40 mg/l), indoleacetic acid (IAA, 0.5 mg/l), kinetin (K, 10 mg/l). sucrose (4%) and agar (0.7%) at pH 5.5. On this medium, a high number of proliferating shoots was obtained. The number of these shoots was increased and their overall development improved by sub-culturing on a MS medium with a reduced concentration of K (5 mg/l) and without the additional amount of sodium dihydrogen phosphate. In these conditions, a 4.5 rate of shoot proliferation was reached after 3 weeks. To induce rooting, the proliferated shoots were transferred to a medium containing half MS, thiamine-HCl (1 mg/l), m-inositol (100 mg/l), ascorbic acid (10 mg/l), naphthaleneacetic acid (NAA, 2 mg/l), sucrose (2%), agar (0.7%) at pH 5.5. In these culture conditions about 84% of plantlets rooted. Cytological analyses performed on root tips of 20 randomly chosen plantlets showed that all the analysed plants contained the diploid number of chromosomes. The plantlets were successfully transferred to soil and the method described seems to be suitable for rapid propagation of globe artichoke.
Publication Year: 1981
Publication Date: 1981-03-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 51
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