Abstract: Mass propagation protocol, callus induction and subsequent plant regeneration in vitro in Sugarcane cultivars, CoS 8436 and CoJ 83 were worked out. Axillary bud induction observed best (80-95%) from field grown (8-12 months old) shoot tip spindle explants within 7-10 days in semi solid MS media supplemented with BAP (0.5 mg/l) and Kn (0.5mg/l), however maximum shoots/explant (4.25 5.75) were observed in MS + BAP (1.5mg/l) + Kn (1.5mg/l). After two cycle serial subculture in semi solid media for establishment, shoot clumps were multiplied in liquid media, provide higher multiplication rate (3.63-4) in MS + BAP (1.0mg/l) + Kn (1.5mg/l) and higher shoot elongation in MS + BAP (0.5mg/l) + Kn (0.5mg/l). In vitro grown shoots/shoot clumps show earlier root induction (5-7 days), higher no of roots and root length in MS+ NAA (5 mg/l) + sugar (70gm/l). Spindle explants show better callus induction response in MS + 2, 4-D (3mg/l) within 14 days in dark and multiplied in reduced/half conc. of auxin. Plantlet regeneration (68-74%) were best obtained from compact nodular, morphogenic calli cultured in MS media supplemented with BAP (0.5mg/l) and Kn (0.5mg/l) cultured in light and show similar responses for shoot multiplication, elongation and rooting.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: dissertation
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Cited By Count: 11
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